TRANSLATIONAL ASPECT OF TUMOR MICROENVIRONMENT IN THE HEAD AND NECK CANCER

This symposium will feature four topics 

Tuula Salo – Session Description:

“The role extracellular matrix in analysing oral cancer cells behavior

Extracellular matrix is an essential component in oral cancer.  In vitro cancer research has lacked human tissue models that mimic the natural tumor microenvironment (TME) matrix. The 3D invasion and growth of carcinoma cells has been investigated with organotypic models composed of type I collagen from rat tail combined with tumor derivatives, such as Matrigel®, from mice. However, these classical methods mix matrices from different species and do not accurately mimic the composition of human tumor extracellular matrix. Our group has developed 3D invasion assays, myoma discs and myogel, derived from human benign uterine leiomyoma tissue. These models contain all essential TME components for in vitro experiments. Myoma assays fit also for co-culture experiments of various TME cells, such as carcinoma associated fibroblasts, macrophages and inflammatory cells. Myogel provides an excellent matrix for analyzing cancer cell migratory, invasive or adhesive properties, as well as potential anticancer drugs. Thus, myoma discs together with myogel offer practical human matrices for translational cancer research purposes.